We have created and ePub3 accessible file and validated the same using the latest ePub checker but the in the Mac OS voice-over is not working in ADE. The navigation panel is read but when we go to the body content we get the Frame 0 being repeated. Do we need to change anything in the opf metadata to get this working. Any advise on this will be very helpful, thanks in advance.
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ADE is a proprietary application developed by Adobe Systems. You need to contact them.
http://www.adobe.com/solutions/ebook/digital-editions.html
Just to confirm, the epub metadata doesn't control operating-system level features like voiceover. If an application does not work with an OS feature, it's a problem with the application.
epubtest.org has reading system evaluations for accessibility, but they don't have an entry for ADE on Macs.